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Solvent tolerant microorganisms and methods of isolation
Published online: Mar 30, 2009
| Geographical region | Europe |
|---|---|
| Subject | Butanol production |
| Published date | Feb 25, 2009 |
| Title | Solvent tolerant microorganisms and methods of isolation |
| Patent Application Number | EP2027254 A1 |
| Assignee | Du Pont, USA |
| Relevance | A method for fermentive production of butanol from carbon sources using genetically engineered Lactobacillus bacteria. |
| Type of claims | Publication |
| Overview | This patent discloses a method of isolation of butanol tolerant microorganism to produce butanol, 2-butanone and ethanol using genetically engineered Lactobacillus comprises butanol biosynthetic pathway or a 2-butanone biosynthetic pathway. Butanol tolerant microorganisms are tolerant to 2.5% w/v 1-butanol when grown on a solid medium at 37°C. The method comprises, A) providing a microbial sample comprising a microbial consortium, B) contacting the microbial consortium with a growth medium comprising a fermentable carbon source until the members of the microbial consortium are growing, C) contacting the growing microbial consortium of step (B) with butanol and D) isolating the viable members of step (C) wherein a butanol tolerant microorganism is isolated. |
| Related patent documents |
US Patent publications
US6358717 - Method of producing butanol using a mutant strain of Clostridium beijerinckii (2002)
Not Listed |
| Patent Document | Full Patent Document [external link] |
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