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Reduction of carbon dioxide emission during isoprene production by fermentation

Published online: Jul 30, 2010

Geographical region	USA
Subject	Production of isoprene
Published date	Jul 1, 2010
Title	Reduction of carbon dioxide emission during isoprene production by fermentation
Patent Application Number	US20100167370 A1
Inventor	Chotani Gopal K.; Nielsen Alex; Sanford Karl J., USA Correspondence Care of Morrison & Foerster LLP, USA
Relevance	Method for producing isoprene using cultured cells via fermentation.
Type of claims	Publication
Overview	This patent discloses a method for producing greater yield of isoprene by cultured cells such as Bacillus subtilis, Pantoea citrea, Aspergillus niger, etc., via fermentation. The method involves cells comprising a heterologous isoprene synthase nucleic acid in operable combination with a promoter. Advantageously, the method results in high productivity, high isoprene purity, and reduces carbon dioxide emissions, and is cost effective. The method comprises, A) culturing cells in culture medium under conditions, wherein carbon source is chosen from glycerol, cell mass, protein, alcohol, and plant-derived oil and B) producing isoprene, wherein the ratio of carbon dioxide emitted per isoprene produced in off gas from the cultured cells is less than 500.
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Patent Document	Full Patent Document [external link]

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