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Process for cell-free production of chemicals

Published online: Jul 30, 2010

Geographical region Europe
Subject Production of organic compounds
Published date Jul 7, 2010
Title Process for cell-free production of chemicals
Patent Application Number EP2204453 A1
Assignee Sued-Chemie AG, Germany
Relevance Process for producing organic compounds from carbon sources using an enzymatic pathway.
Type of claims Publication
Overview This patent discloses an enzymatic process for producing organic compounds such as ethanol, butanol, isobutanol, etc., from carbon sources using cell free enzyme subsystem. The process involves converting glucose to pyruvate as an intermediate product, wherein no net production of ATP occurs. Advantageously, the process enables improved yield, maximum catalystic efficiency and simplified product purification. The process comprises, A) producing enzymes for the conversion of a carbon source into a chemical including target organic compound using microbial cells, B) releasing target enzymes from the microbial cells used in step (A), that are combined with the release of cofactors and with inactivation of non-target enzyme activities, C) bringing the target enzymes of step (B) in contact with the carbon source under conditions suitable for the conversion of the carbon source into the target chemical and D) separating the target chemicals from the reaction mixture.
Related patent documents US Patent publications

Not Listed

Foreign patent publications

WO2008052596 - Process for the biological production of n-butanol with high yield (2008)
WO2008006038 - Methods and compositions for butanol production (2008)

Patent Document Full Patent Document [external link]

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