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Solventless extraction process

Published online: Apr 30, 2008

Geographical region	USA
Subject	Microbial oil extraction
Issue date	Apr 1, 2008
Title	Solventless extraction process
Patent Number	US7351558 B2
Patent Application Number	US2004229325 A1
Assignee	Martek Biosciences Corp, USA
Relevance	A process of extracting, recovering, isolating microbial oil without using organic solvent.
Type of claims	Grant
Overview	This patent discloses a process for obtaining lipid, in particular oil containing omega-3 highly unsaturated fatty acids like DHA from microorganisms. The method advantageously does not use an organic solvent and hence reducing the overall production cost of lipid extraction. Advantageously, the present invention allows fermentation of microorganisms and isolation of lipids carried out in a single vessel. The method includes, A) lysing cells of the microorganisms to produce a lysed cell mixture, b) treating the lysed cell mixture to produce a phase separated mixture comprising a heavy layer and a light layer, c) separating the heavy layer from the light layer and d) obtaining the lipid from the light layer.
Related patent documents	US Patent publications US4504473 - Pyridine soluble extract of a microorganism (1985) US4720456 - Trehalose lipid tetraesters (1988) US4792418 - Method of extraction and purification of polyunsaturated fatty acids from natural sources (1988) US5130242 - Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids (1992) US5179012 - Process for the production and extraction of antioxidants from a micro-organism culture (1993) US5340594 - Food product having high concentrations of omega-3 highly unsaturated fatty acids (1994) US5539133 - Process for extracting lipids with a high production of long-chain highly unsaturated fatty acids (1996) US5928696 - Process for extracting native products which are not water-soluble from native substance mixtures by centrifugal force (1999) US6166231 - Two phase extraction of oil from biomass (2000) US6255505 - Microbial polyunsaturated fatty acid containing oil from pasteurised biomass (2001) Foreign patent publications WO0153512 - Solventless extraction process (2001) WO9803671 - Process for preparing docosahexaenoic acid and docosapentaenoic acid (1998)
Patent Document	Full Patent Document [external link]

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