Production and secretion of glucose in photosynthetic prokaryotes (Cyanobacteria)
Published online: Apr 30, 2008
| Geographical region | USA |
|---|---|
| Subject | Production of glucose |
| Published date | Apr 10, 2008 |
| Title | Production and secretion of glucose in photosynthetic prokaryotes (Cyanobacteria) |
| Patent Application Number | US20080085520 A1 |
| Assignee | Board of Regents, The University of Texas, USA |
| Relevance | Compositions, methods, systems and kits for the production of microbial cellulose using Cyanobacteria that includes an exogenous cellulose operon sufficient to express bacterial cellulose. |
| Type of claims | Publication |
| Overview | This patent discloses a method of producing a photobiomass such as monosaccharides, disaccharides, oligosaccharides or polysaccharides by modifying a Cyanobacterium with a portion of an exogenous bacterial cellulose operon sufficient to express and produce extracellular glucose. Advantageously, the Cyanobacteria do not produce lignin along with the production of the cellulose and other saccharides that can be used as a feed-stock for fermentation that convert the saccharides into ethanol or other synfuels. The method also eliminates a variety of processing steps including, milling, bulk-fiber separations, recovery or treatments for the control or elimination of lignin, water removal, distillation and burning of unwanted byproducts. Also it provides a novel CO2 fixation method for the recycling of environmental greenhouse gases. The method comprises, A) modifying a Cyanobacterium with a portion of an exogenous bacterial cellulose operon sufficient to express and produce extracellular glucose, B) growing the Cyanobacteria under conditions that promote extracellular glucose production and C) exposing the Cyanobacteria to an acidic condition, wherein the acid increases extracellular glucose production and optionally that the viable cells are returned to the growth medium after glucose harvest for continued production of cells, biomass, cellulose or glucose. |
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| Patent Document | Full Patent Document [external link] |
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