skip
REGISTER ON BIOFPR.COM

Already registered? LOGIN.

Browse By Subject:
Archived Content:
Home > Patent Intelligence >

Patent Intelligence Article

Process for the fermentative preparation of L-amino acids with amplification of the TKT gene

Published online: Nov 16, 2007

Geographical region Europe
Subject Preparation of L-lysine
Issue date Oct 3, 2007
Title Process for the fermentative preparation of L-amino acids with amplification of the TKT gene
Patent Number EP1179084 B1
Patent Application Number EP1179084 A1
Assignee Degussa, Denmark; NAT University of Ireland, Ireland
Relevance An improved process for the preparation of L-lysine using Coryneform bacteria.
Type of claims Grant
Overview This patent discloses a process for the fermentative preparation of L-lysine using Coryneform bacteria in which the nucleotide sequence which codes for the enzyme transketolase (tkt gene) is amplified, in particular over-expressed. The process involves, A) amplification of tkt gene coding for transketolase in Coryneform bacterium, B) fermentation of the bacterium in which tkt gene is amplified in a medium, C) concentration of L-Lysine in the medium or in the cells of the Coryneform bacterium and D) isolation of L-Lysine.
Related patent documents US Patent publications

US5175108 A - Plasmids from Corynebacterium glutamicum and plasmid vectors derived therefrom (1992)
US5158891 A - Plasmid containing a gene for tetracycline resistance and DNA fragments derived therefrom (1992)

Foreign patent publications

JP09224661 A - Glucose-6-phosphate dehydrogenase and DNA capable of coding the same (1997)
JP9224662 A - 6-Phosphogluconate dehydrogenase and DNA capable of coding the same (1997)
JP10229891 A - Production of malonic acid derivative (1998)
DE19947791 A - Nucleotide sequences coding for the eno gene (2001)
DE19950409 A - Coryneform Bacteria with a deletion of Phosphoenolpyruvate-carboxykinase and their use (2001)
EP1087015 A - Cloning and uses of a novel nucleotide sequence coding for glucose-6-phosphate isomerase (gpi) from bacteria (2001)
EP0472869 A - Plasmids of Corynebacterium glutamicum and derived plasmid vectors (1992)
WO9615246 A - DNA which regulates gene expression in Coryneform bacteria (1996)
DE19951975 A - Nucleotide sequence encoding Corynebacterium poxb-gene and its use in the preparation of l-lysine (2001)
EP0435132 B - Process for the fermentative production of amino acids, especially L-lysine, microorganisms suitable therefor and recombinant DNA (1991)
DE19931314 A - L-lysine producing Coryneform bacteria and methods for the production of L-lysine (2001)

Patent Document Full Patent Document [external link]

Tag this article

Please register and login to tag our features, news and patent summaries.

Displaying 4 keywords used to tag this article:
  • L-lysine  
  • fermentation  
  • isolation  
  • amino acids  

Comment on this article

Please register and login to comment on our features, news and patent summaries.

This article has not been commented on. Be the first to comment on this article.