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A method to produce succinic acid from raw hydrolysates

Published online: Jul 30, 2010

Geographical region Europe
Subject Production of succinic acid
Issue date Jul 7, 2010
Title A method to produce succinic acid from raw hydrolysates
Patent Number EP1751292 B1
Patent Application Number EP1751292 A1
Assignee UT-Battelle LLC; The University of Chicago; Uchicago Argonne LLC, USA
Relevance Method for producing succinic acid from raw hydrolysates using bacterial mutants.
Type of claims Grant
Overview This patent discloses a method for producing succinic acid from industrial grade hydrolysates such as lignocellulosic hydrolysate or corn-derived sugar solutions, using bacterial mutants chosen from Escherichia coli, Klebsiella, Erwinia, Lactobacillus, etc. The method involves supplying an organism that contains mutations for the genes ptsG, pflB and ldhA, allowing the organism to accumulate biomass and metabolize the hydrolysate. Advantageously, the method enables large scale production of succinic acid. The method comprises, A) supplying an organism from an Escherichia coli strain selected from AFP 184 (ATCC PTA 5132), AFP 400 (ATCC PTA 5583) and AFP 404 (ATCC PTA 5133), B) allowing the organism to accumulate biomass in an aerobic atmosphere, and C) allowing the organism to metabolize the hydrolysate, wherein the method is made continuous with the addition of feedstock and thereby producing succinic acid.
Related patent documents US Patent publications

US20030017559 A1 - Method to produce succinic acid from raw hydrolysates (2003)

Foreign patent publications

WO2004043881 - A method to produce succinic acid from raw hydrolysates (2004)

Patent Document Full Patent Document [external link]

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